|（1.Guangdong Vocational Institute of Sports，Guangzhou 510663，China；
2.School of Physical Education，South China Normal University，Guangzhou 510006，China；3.Guangzhou Sport University，Guangzhou 510500，China）
Abstract: In order to observe the effects of aerobic exercising combined with glutamine (Gln) supplement on the serum glucagon like peptide 1 (GLP-1), insulin (INS) and fasting blood glucose (FBG) levels of rats with type 2 diabetes mellitus (T2DM), the authors divided 60 male SD rats ((179.8±19.2) g) randomly into a healthy control group (group C, 26 rats) and a diabetic model establishment ground (group D, 34 rats). The rats in group C were fed with common feed, while the rats in group D were fed with high fat feed. 4 weeks later, the rats in group D were in-jected with 35 mg/kg of streptozotocin (STZ) to induce T2DM. After model formation, the rats in the two groups were further divided into calm groups (CQ, DQ), exercising groups (CE, DE), Gln groups (CG, DG) and exercising plus Gln groups (CEG, DEG) randomly. The rats in the exercising groups did a 6-week swimming exercise. The rats in the Gln groups were fed with Gln a mass fraction of 2% L. Abdominal aorta blood was taken to test FBG, insu-lin and GLP-1 levels. Results: 6-week swimming exercise or Gln supplement significantly increased the GLP-1 and insulin concentrations of the rats in group D, significantly decreased FBG value, significantly improved the symptom of polyphagia of the rats in group D, but had no significant effect on the body weight. 6-week exercising significantly increased the insulin level of the rats in group C, significantly decreased their FBG value, had no sig-nificant effect on GLP-1; 6-week Gln supplement significantly decreased the food intake of the rats in group C, had no significant effect on their FBG, insulin and GLP-1 levels. When exercising was combined with Gln supplement, the control of FBG, the increase of plasma GLP-1, the increase of insulin level and the improvement of consumption symptom of the rats in group D were more significant than those showed when exercising or Gln supplement worked separately, but there was no significant difference in the effect on the body weight; the effect of the combi-nation of exercising and Gln supplement on GLP-1 of the rats in group C was more significant than the effect pro-duced when exercising or Gln supplement worked separately, but had no significant effect on FGB, insulin and body weight. The results indicated the followings: long term aerobic exercising or Gln supplement can increase the GLP-1 level of rats with T2DM, increase their insulin secretion, lower their blood sugar, and improve their poly-phagia symptom; when exercising is combined with Gln supplement, it can lower the blood sugar of rats with T2DM, and its effect on increasing their GLP-1 and insulin levels is more significant than the effect produced when exercising or Gln supplement works as a single factor.
Key words: sports biochemistry；Type 2 diabetes mellitus；aerobic exercise；glutamine；glucagon-like peptide 1；insulin