|（Department of Physical Education，Henan University of Economics and Law，Zhengzhou 450002，China）
Abstract: In order to probe into the effects of endurance training on the gene expression of the aerobic respiration regulating path of diabetic GK rats, the author divided 12 rats randomly into 2 groups: a calm group (GKC, n=6) and an endurance training group (GKE, n=6), put group GKC into treadmill training for 6 weeks, took out the gastrocnemius within 24-48h after the last time of training, tested the mRNA transcription of p53, SCO2 and COXII by means of real-time PCR, as well as the protein expression of p53, SCO2 and COXII by means of western blot, and revealed the following findings: 1) the gene transcription and protein expression levels of p53 of the rats in group GKE were sig-nificantly lower than those of the rats in group GKE; 2) comparing group GKE with group GKE, there were no signifi-cation differences in terms of gene transcription and protein expression levels; 3) comparing group KF with group GKC, there was no signification difference in terms of COXII gene transcription level, but the COXII protein expres-sion level of the rats in group GKE was very significantly lower than that of the rats in group GKC. The findings indi-cate that endurance training cannot significantly boost the aerobic respiration ability of the mitochondria of diabetic rats. The significant lowering of the COXII protein expression level seems to show that the endurance training model in this experiment was harmful to their aerobic respiration axis, however, as cautiously figured by considering the result of the significantly lowering of P53 gene expression, the cause may be as follows: since the p53 gene expression level was unable to continue to maintain the normal homeostasis as affected by the course of diabetes, the lowering of its gene expression ability was extremely likely for boosting cell survival, or probably for delaying the dying or aging process of the sick cells of the GK rats. Under the condition of sickness, a more important function of cells is probably not to en-hance moving ability, but to accumulate all the strengths to promote cell survival.
Key words: sports biochemistry；endurance training；aerobic respiration；diabetes；rat