| （School of Physical Education，South China Normal University，Guangzhou 510006，China） Abstract: In order to probe into the influence of heat shock protein 70 induced by the joint intervention of high temperature pretreatment and one-time acute exercising with different intensities on free radicals and Ca2+-ATPase in the hippocampus area of rats, the authors divided 48 SD 3-month old rats into 6 groups: a room temperature calm group, a room temperature 15m/min exercising group, a room temperature 27m/min exercising group, a high tem-perature calm group, a high temperature 15m/min exercising group, and a high temperature 27m/min exercising group. 24h after the rats in high temperature groups (8 in each group) were pretreated by high temperature, they did a 60min one-time acute exercise together with the rats in other exercising groups, and immediately after that, hot shock protein Hsp70 and MDA contents as well as SOD and Ca2+-ATPase activities in the hippocampus were sam-ple taken and tested. The results were as follows: comparing the rats in the room temperature 27m/min exercising group with the rats in the room temperature calm group, the level of expression of Hsp70 in the hippocampus area rose significantly (P<0.05); the levels of expression of Hsp70 of the rats in groups pretreated by high temperature were significantly higher than those of the rats in room temperature groups (P<0.05); as compared with the rats in room temperature exercising groups, the SOD activity of hippocampus of the rates in exercising groups pretreated by high temperature increased extremely significantly (P<0.01); the MDA contents of the rats in high temperature groups decreased as exercise intensity increased, but showed no significant difference; the Ca2+-ATPase activities of the rats in groups pretreated by high temperature were all significantly higher than those of the rats in room tem-perature groups (P<0.05). The results indicated the followings: a lot of Hsp70 expression induced by high tempera-ture pretreatment can boost the SOD activity of hippocampus during exercising, lower MDA content, and boost Ca2+-ATPase activity. It is suggested that Hsp70 can reduce the damage to the hippocampus area cased by free radi-cals by enhancing antioxidation ability, thus it is provided with certain significance in protecting the nervous system.
Key words: sports biochemistry；heat stress protein 70；high temperature pretreatment；hippocampus area；free radical；Ca2+-ATPase；rats |
